Tuning is calibrating for optimal performance at a given environment. Many ECUs have feedback & re-calibrate on a small scale one near perfect is set through a dyno usually. Beyond all of that at any race there is anything from the car owner/driver to a full crew of data analyst engineers that continually observe reading from the electronics & adjust to increase performance. These adjustments are not done at steady state on a dyno. To the OP, yes is the short answer. What AEM are you refering to the older Series or the newer Infinity? There is a base tune that is best used to start the car & check that all systems are functioning properly. From there you can either road tune or dyno tune. I would recommend spending a couple hours on a dyno. The ECU may & you can also continue to refine what ever your base tune is after that if you desire or need. When a new scope is sighted in on a rifle do you use a controlled safe environment like a target in a shooting range (dyno) or a go to the desert & aim at a can in the open elements (road tuning)? Both can work & get you pretty accurate with enough time, but one is better than the other.
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